Tillering dynamics of Alexandergrass pasture under nitrogen fertilization / Dinâmica do perfilhamento da pastagem de Papuã sob fertilização nitrogenada

This study was carried out to evaluate the effect of nitrogen fertilization on tillering dynamics of Alexandergrass (Urochloa plantaginea (Link) Hitch) grazed by beef heifers in a rotational stocking grazing method. The experiment was conducted in a completely randomized design following a repeated measure arrangement, three levels of nitrogen (Zero, 150 and 300kg ha-1) and two area repetition. The classification of tillers into categories (basal and axillary) showed a greater number of basal tillers, due to their higher appearance rate, in the absence of nitrogen fertilization. Nitrogen fertilization promoted higher axillary tiller density. The use of nitrogen promotes the renewal of axillary tillers of Alexandergrass, without compromising the stability of the tiller population.(AU)

Esse estudo foi conduzido para avaliar o efeito da fertilização nitrogenada no perfilhamento do Papuã (Urochloa plantaginea (Link) Hitch) pastejado por novilhas de corte em um método de lotação rotacionada. O delineamento foi inteiramente casualizado com arranjo de medidas repetidas, três níveis de nitrogênio (Zero, 150 e 300kg ha -1 ) e duas repetições de área. A classificação dos perfilhos em categorias (basais e axilares) mostrou um maior número de perfilhos basais, devido à sua maior taxa de aparecimento, na ausência de adubação nitrogenada. A fertilização nitrogenada promoveu maior densidade de perfilhos axilares. O uso de nitrogênio promove renovação dos perfilhos axilares no Papuã, sem comprometer a estabilidade da população de plantas.(AU)

Análise bioeconômica do farelo de arroz na recria de bezerras de corte em azevém / Bioeconomics analyses of rice brain use for reaning of beef heifers on Italian ryegrass pasture

Objetivou-se avaliar a economicidade, o desempenho produtivo e o desenvolvimento das estruturas corporais relacionadas ao trato reprodutivo de bezerras de corte mantidas exclusivamente em pastagem de azevém (Lolium multiflorum Lam.) ou em pastagem de azevém recebendo 0,5 e 1,0% do peso corporal (PC) de farelo de arroz integral (FAI) como suplemento. O método de pastejo foi o rotativo, e o intervalo entre pastejos foi estabelecido considerando-se a soma térmica necessária para a emissão de 1,5 folha de azevém (187,5ºC). O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo. O ganho médio diário das bezerras não diferiu entre os sistemas alimentares. A taxa de lotação e o ganho de peso por área foram maiores quando as bezerras receberam 1,0% do PC de farelo de arroz integral. A maior margem bruta foi observada quando as bezerras receberam 0,5% de FAI. O custo variável obtido para os diferentes sistemas alimentares apresentou comportamento crescente em função dos níveis de FAI, superando o uso exclusivo do azevém em 49,4% e 81,8%. O peso corporal final, o escore de condição corporal e o escore do trato reprodutivo não diferiram entre os sistemas alimentares. Considerando-se um animal adulto com peso corporal médio de 450kg, as bezerras ao início do experimento apresentaram 34% do peso corporal adulto e peso corporal final médio de 56% do peso adulto. A utilização de níveis de farelo de arroz integral (FAI) na recria de bezerras de corte sob pastejo rotativo em azevém não modifica o ganho médio diário e o escore do trato reprodutivo. O fornecimento de 1% de FAI mostrou efeito substitutivo, proporcionando um incremento de 31,2% na taxa de lotação e de 38,3% no ganho por área. O sistema alimentar AZ0,5 permitiu uma maior margem bruta; já o retorno financeiro direto foi positivo em todos os sistemas alimentares, com melhor retorno calculado para o uso exclusivo do azevém.(AU)

The experiment was carried out with the objective of evaluating the gross margin, the productive and reproductive performance of beef heifers kept exclusively on ryegrass pasture (Lolium multiflorum Lam.) or ryegrass receiving 0.5 and 1.0% of body weight (BW) of whole rice bran as supplement. The grazing method was the rotational and the interval between grazings was set by the thermal sum required for the appearing of 1.5 ryegrass leaf (187.5ºC). The experimental design was completely randomized with repeated measures. Heifers average daily gain did not differ between the feeding systems. The stocking rate and weight gain per area were higher when heifers received 1.0% BW of whole rice bran. The higher gross margin was observed when heifers received 0.5% BW. The variable cost obtained for the different feeding systems showed increasing behavior depending on the WRB levels, surpassing the exclusive use of ryegrass in 49.4% and 81.8%. The final body weight, body condition score and reproductive tract score did not differ between the feeding systems. Considering an adult animal with an average body weight of 450kg, the heifers at the beginning of the experiment showed 34% of mature body weight and final average body weight of 56% of adult weight. The use of rice bran levels (FAZ) in the rearing of beef heifers under rotational grazing on ryegrass does not change the average daily gain and the reproductive tract score. The supply of 1% FAI showed substitutive effect, providing an increase in capacity of 31,2% and 38.3% rate of the gain per area. The food system AZ0,5 allowed a higher gross margin, as the economic return was positive in all food systems, with better return calculated for the exclusive use of ryegrass.(AU)

Moraxella bovis, Moraxella ovis and Moraxella bovoculi: biofilm formation and lysozyme activity.

AIMS: This study aimed to verify the formation of biofilms by Moraxella bovis, Moraxella ovis and Moraxella bovoculi isolates from ruminants. In addition, the lysozyme activity against the isolates of M. bovis, M. ovis and M. bovoculi in free form and in biofilms was determined. METHODS AND RESULTS: In this study, 54 isolates of Moraxella sp. obtained from bovine and ovine clinical samples were evaluated in vitro for capacity of biofilm formation and lysozyme susceptibility in planktonic and sessile cells. In addition, biofilms produced by four Moraxella sp. isolates were visualized under scanning electron microscope (SEM). It was possible to demonstrate, for the first time, the ability to form biofilms by M. ovis and M. bovoculi. The isolates of Moraxella sp. have the capacity to form biofilms in different intensities, varying among weak, moderate and strong. It was verified that the lysozyme shows activity on Moraxella sp. in planktonic form. However, on biofilms there was a reduction in the production, but without impairing its formation, and on consolidated biofilms the lysozyme did not have the capacity to eradicate the preformed biofilms. CONCLUSIONS: This work shows the capacity of biofilm formation by Moraxella sp. of veterinary importance. The lysozyme susceptibility of Moraxella sp. in planktonic form shows that this enzyme has bacteriostatic activity on this micro-organism and it reduced the production of biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY: Based on the results, it is possible to infer that the biofilm formation capacity by Moraxella sp. and the resistance to lysozyme concentrations equal to or greater than the physiological levels of the ruminant tear may be linked not only to the capacity to colonize the conjunctiva, but also to remain in this place even after healing of the lesions, being a reservoir of Moraxella sp. in a herd.

Coinfecções por Leishmania infantum, Neospora caninum e Toxoplasma gondii em cães necropsiados da região central do Rio Grande do Sul, Brasil / Coinfections by Leishmania infantum, Neospora caninum and Toxoplasma gondii in necropsied dogs from the central region of Rio Grande do Sul, Brazil

O objetivo do presente trabalho foi determinar a presença de anticorpos para Leishmania infantum, Neospora caninum e Toxoplasma gondii, por meio da reação de imunofluorescência indireta (RIFI), em cães (n=78) provenientes da região central do Rio Grande do Sul, necropsiados no Hospital Veterinário da Universidade Federal de Santa Maria (UFSM), bem como avaliar os dados epidemiológicos, sazonais e anátomo-histopatológicos. Do total de animais avaliados, 67,9% (53/78) apresentaram soropositividade para ao menos um agente. A ocorrência de anticorpos para L. infantum, N. caninum e T. gondii foi de 33,3 (26/78), 37,1 (29/78) e 43,5% (34/78), respectivamente. Detectaram-se monoinfecções em 9,4% (5/53) para L. infantum, 18,8% (10/53) para N. caninum e 20,7% (11/53) para T. gondii. As coinfecções foram observadas em 27/53 (50,9%) dos animais. As infecções ocorreram independentemente de idade, sexo, procedência ou raça (P>0,05). Não se verificaram lesões anatomo-histopatológicas relacionadas aos agentes pesquisados, caracterizando-os como animais assintomáticos. Os resultados confirmaram a exposição de cães a esses protozoários na região central do RS e, em especial, demonstraram a circulação do agente causador da leishmaniose em uma área considerada indene para a enfermidade.(AU)

The present paper is aimed to determine the presence of antibodies for Leishmania infantum, Neospora caninum and Toxoplasma gondii by indirect immunofluorescence (IIF) in dogs (n=78) from the central region in the state of Rio Grande do Sul necropsied in the Veterinary Hospital from Universidade Federal de Santa Maria (UFSM). The data was evaluated regarding epidemiological, anatomic, and histopathologic findings. Of the total animals evaluated, 67.9% (53/78) showed seropositivity for at least one agent. The occurrence of antibodies to L. infantum, N. caninum and T. gondii was 33.3% (26/78) 37.1% (29/78) and 43.5% (34/78), respectively. The mono infections were detected in 9.4% (5/53) of L. infantum, 18.8% (10/53) for N. caninum and 20.7% (11/53) T. gondii. The coinfections occurred in 50.9% (27/53) of animals. There were not anatomical and histopathological lesions regarding these surveyed agents, characterizing them as subclinical animals. The results confirmed the exposition of dogs to these protozoa in the central region of the RS, highlighting the circulation of the causer agent of leishmaniasis in an area considered harmless for the disease.(AU)

Desempenho de bezerras de corte em pastagem de azevém recebendo farelo de arroz com ou sem monensina / Beef heifers performance in ryegrass pasture supplemented with rice bran with or without monensin

Foi estudado o desempenho de bezerras de corte em pastagem de azevém (Lolium multiflorum Lam.), com animais exclusivamente em pastejo ou recebendo farelo de arroz integral (FAI) em nível de 0,8% do peso corporal, associado ou não à monensina. O método de pastejo foi contínuo com número variável de animais. O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo, com três tratamentos e três repetições de área. O uso de FAI associado ou não à monensina não expressou alteração na área pélvica das bezerras, na taxa de lotação e no ganho de peso por área. As bezerras que receberam FAI, com ou sem monensina, apresentaram maior ganho diário de peso corporal, peso corporal, escore de condição corporal e relação peso corporal:altura. O fornecimento de suplementação energética para bezerras de corte é uma alternativa viável em sistemas de produção que visam à redução da idade ao primeiro acasalamento.(AU)

The experiment was carried out with the objective of evaluating beef cattle heifer performance when exclusively grazing Italian ryegrass (Lolium multiflorum Lam.) pasture or supplemented with whole rice bran (WRB), equivalent to 0.8% of body weight, associated or not with monensin. The grazing method was "put-and-take" stocking, in a completely randomized experimental design, with replicated measures over time, with three treatments and three replications of area. The use of WRB, associated or not with monensin, did not affect heifers' pelvic area, stocking rate and area weight gain. Animals fed WRB, with or without monensin, showed greater average daily gain, body weight, body condition score, and body weight:height ratio. Energetic supplementation for beef heifers is one viable option in production systems seeking age reduction at the first mating.(AU)

Forage intake and performance of heifers fed rice bran on Alexandergrass pasture / Ingestão de forragem e performance de bezerros alimentados com farelo de arroz integral em pasto de papuã

he study was carried out to evaluate the forage intake and performance of beef heifers from 15 to 18 months of age, grazing on Alexandergrass (Urochloaplantaginea (Link) Hitch) fed rice bran (RB; 0, 0.5 and 1.0% body weight (BW)). The experiment consisted of a completely randomized design with repeated measurement arrangement. Rice bran increased the total dry matter (DM) intake, total crude protein intake and total digestible nutrients intake. Heifers that received 1.0% BW of RB reduced forage DM intake, increasing by 18% stocking rate (SR) and by 27% BW gain per area in comparison with the system exclusively on pasture. In the system in which the heifers were fed 0.5% BW of RB, SR and gain per area were similar to other systems. In this feeding system, we observed average daily gain 18% higher than when heifers were exclusively on pasture and gain similar to that observed when using 1.0% BW of RB. In Alexandergrass pasture it is recommended to supply 0.5% BW of RB for beef heifers from 15 to 18 months of age, to promote better productive responses compared to heifers exclusively on pasture and similar to those observed when supplying 1.0% BW of RB.(AU)

O presente trabalho foi desenvolvido com o objetivo de avaliar o consumo de forragem e o desempenho de novilhas de corte dos 15 aos 18 meses de idade, em pastejo em papuã (Urochloaplantaginea(Link) Hitch), recebendo farelo de arroz integral (FAI) como suplemento (0, 0,5 e 1,0% do peso corporal (PC)). O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo. O uso de FAI aumentou o consumo total de matéria seca (MS), o consumo total de proteína bruta e de nutrientes digestíveis totais em relação às novilhas exclusivamente em pastejo. As novilhas que receberam 1,0% do PC de FAI reduziram o consumo de MS da forragem, aumentando em 18% a taxa de lotação (TxLot) e em 27% o ganho de PC por hectare (GPA) em comparação com o sistema exclusivamente a pasto. No sistema em que as novilhas receberam 0,5% do PC de FAI, a TxLot e o GPA foram similares aos demais sistemas. Nesse sistema alimentar, foi observado GDM 18% superior em relação às novilhas exclusivamente em pastejo e ganho similar ao uso de 1,0% do PC de FAI. Em pastagem de papuã, é recomendado o uso de 0,5% do PC de FAI para novilhas de corte dos 15 aos 18 meses de idade, por promover respostas produtivas superiores em relação às novilhas exclusivamente em pastejo e semelhantes às observadas quando fornecido 1,0% do PC de FAI.(AU)

Padrões de deslocamento de bezerras de corte recebendo suplemento em pastagem de azevém / Displacement patterns of beef heifers receiving supplement in Italian ryegrass pasture

O objetivo deste trabalho foi avaliar o tempo de permanência, o número de bocados nas estações e o deslocamento entre estações alimentares por bezerras de corte em pastagem de azevém (Lolium multiflorum Lam.) e recebendo farelo de arroz integral como suplemento em três níveis (0,0; 0,5 e 1,0% PC). O método de pastejo foi rotacionado, com número variável de animais. O período de descanso foi estabelecido pela soma térmica (ST) de 187,5ºC, equivalente à ST necessária para a emissão de 1,5 folhas de azevém. O delineamento experimental foi inteiramente ao acaso, com medidas repetidas no tempo, três níveis de suplemento e seis ciclos de pastejo. O tempo de permanência em cada estação alimentar (9,4s) foi semelhante para bezerras exclusivamente a pasto e as que receberam 1,0% PC de suplemento. As bezerras que receberam 0,5% PC de suplemento permanecem 1,1s a mais na estação alimentar quando comparadas com as bezerras nos demais níveis. O deslocamento entre estações alimentares (1,7 passos) e o número de bocados em cada estação alimentar (sete bocados) foram semelhantes independentemente do nível de farelo de arroz fornecido. As bezerras, ao receberem 0,5% PC de farelo de arroz, são mais dependentes da presença de lâminas foliares no estrato pastejável do dossel.(AU)

The aim of this study was to evaluate the length of stay and number of bites in the feeding stations and displacement between feeding stations for beef heifers on ryegrass pasture (Lolium multiflorum Lam.) and receiving rice bran as a supplement in three levels (0.0; 0.5 and 1.0% BW). The grazing method was rotational with variable number of animals. The interval between grazing was set by the thermal sum (TS) of 187.5ºC equivalent to TS necessary for externalization of 1.5 leaf lamina of ryegrass. The experimental design was completely randomized with repeated measures on time, three levels of supplement and six grazing periods. The stay time at each feeding station (9.4 sec.) was similar for heifers exclusively on pasture and receiving 1.0% supplement BW. Heifers that received 0.5% supplement BW remained 1.1 sec. longer at the feeding station than heifers on other supplement levels. Displacement between feeding stations (1.7 steps) and the number of bites in each feeding station (7 bites) were similar regardless of the supplied rice bran level. Heifers receiving 0.5% rice bran BW are more dependent on the presence of leaf blades in grazeable stratum of canopy.(AU)

Uso de suplementos para acasalamento de bezerras Angus aos 14 meses de idade / Use of supplements for mating Angus heifers at 14 months

O experimento foi conduzido com o objetivo de avaliar o desenvolvimento de novilhas de corte em três sistemas alimentares: exclusivamente em pastagem de azevém (Lolium multiflorum Lam.) ou em pastagem de azevém e recebendo 0,93% do peso corporal de grão de milho inteiro ou grão de aveia branca como suplemento. O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo. A utilização da pastagem de azevém, com ou sem uso de grão de milho ou aveia como suplemento, possibilita desenvolvimento reprodutivo para que as bezerras sejam acasaladas aos 14 meses de idade. O peso corporal ao início da utilização da pastagem é determinante para que as bezerras atinjam adequado desenvolvimento reprodutivo no início da estação de acasalamento.(AU)

The experiment was carried out to evaluate the development of beef heifers in three feeding systems: exclusively on ryegrass pasture (Lolium multiflorum Lam.) or in ryegrass pasture and receiving 0.93% of body weight of corn whole grain or oats grain as a supplement. The experimental design was completely randomized with repeated measures in time. The utilization of ryegrass pasture, with or without the use of corn or oats grain as a supplement, provides reproductive development of heifers in order for them to be mated at 14 months of age. The body weight at the beginning of pasture utilization is crucial for heifers to reach adequate reproductive development at the beginning of the mating season.(AU)

Canopy structure, ingestive behavior and displacement patterns of beef heifers grazing warm-season pastures / Estrutura do dossel, comportamento ingestivo e padrões de deslocamento de novilhas de corte em pastagens de estação quente

Analyses in a data set of six experiments (n=436) was performed with the aim of characterizing canopy structure and forage intake patterns of beef heifers grazing on Pearl millet (Pennisetum americanum), Alexandergrass (Urochloa plantaginea) and Coastcross (Cynodon dactylon). Forage and leaf lamina mass were similar among species, 3001.4 and 668.1kg of DM/ha, respectively, while leaf:stem ratio of canopy vertical strata was different. Intake rate (12g DM/minute), bite mass (0.343g DM/bite) and bite rate (36.6bites per minute) were similar in Pearl millet and Alexandergrass. In Pearl millet, neutral detergent fiber content (56.1%) in forage as grazed, grazing time (518.9 minutes/day) and displacement rate (8.8 steps/minute) were lower. Increased grazing time (639 minutes/day) represented the main compensatory mechanism for smaller bite masses (0.234g DM/bite) and reduced intake rates (8.8 grams of DM/minutes) in Coastcross. Bite rate variations happened as a response to constraints imposed by canopy structure. Surface utilization on Alexandergrass and Coastcross was increased by greater number of feeding stations visited and displacement rate. Ingestive behavior components of beef heifers are affected by differences in the canopy structure of Pearl millet, Alexandergrass and Coastcross.

Foi realizada a análise conjunta dos resultados de seis experimentos (n=436) com o objetivo de caracterizar a estrutura do dossel e os padrões de ingestão de forragem por novilhas de corte em pastagem de milheto (Pennisetum americanum), papuã (Urochloa plantaginea) e coastcross (Cynodon dactylon). As massas de forragem e de lâminas foliares foram similares entre as três espécies forrageiras, 3001,4 e 668,1kg/ha de MS, respectivamente, enquanto a relação lâmina:colmo do perfil vertical do dossel diferiu. A taxa de ingestão (12g MS/minuto), a massa de bocados (0,343g MS/bocado) e a taxa de bocados (36,6 bocados/minuto) foram similares no milheto e no papuã. O conteúdo de fibra em detergente neutro (56,1%) na forragem da simulação do pastejo, o tempo de pastejo (518,9 minutos/dia) e a taxa de deslocamento (8,8 passos/minuto) foram menores no milheto. O incremento no tempo de pastejo (639 minutos/dia) representou o mecanismo compensatório para a menor massa de bocados (0,234g MS/bocado) e para a reduzida taxa de ingestão (8,8 gramas MS/minuto) no coastcross. Variações na taxa de bocado ocorrem como resposta às limitações impostas pela estrutura do dossel. A exploração da área da pastagem de papuã e de coastcross foi aumentada pelo maior número de estações alimentares visitadas e pela maior taxa de deslocamento. Os componentes do comportamento ingestivo de novilhas de corte são afetados por diferentes estruturas no dossel de milheto, papuã e coastcross.

Cardiac toxoplasmosis after heart transplantation diagnosed by endomyocardial biopsy.

We describe a case of cardiac toxoplasmosis diagnosed by routine endomyocardial biopsy in a patient with trimethoprim-sulfamethoxazole (TMP-SMX) intolerance on atovaquone prophylaxis. Data are not available on the efficacy of atovaquone as Toxoplasma gondii prophylaxis after heart transplantation. In heart transplant patients in whom TMP-SMX is not an option, other strategies may be considered, including the addition of pyrimethamine to atovaquone.

A systematic review of pre-operative anaemia and blood transfusion in patients with fractured hips.

We systematically reviewed the observational associations of anaemia with outcomes and the effects of interventions to increase haemoglobin concentrations following hip fracture in older people. Anaemia on hospital admission was associated with increased mortality, relative risk 1.64 (95% CI 1.47-1.82), p < 0.0001. After adjustment for co-morbidities, the association of anaemia with increased mortality remained in four of eight observational studies. There was no association of postoperative transfusion with mortality after adjusting for covariates. Transfusion at 80 g.l(-1) vs 100 g.l(-1) increased acute myocardial infarction, relative risk 1.67 (95% CI 1.01-2.77), p = 0.05. Transfusion threshold was not associated with differences in other outcomes. There were insufficient high-quality studies to inform pre-operative blood transfusion or the use of peri-operative iron or erythropoietin. Studies for most interventions recruited too few participants to determine effects on infections, mortality or function.

Serum sickness after treatment with rabbit antithymocyte globulin in a heart transplant recipient with previous rabbit exposure.

Antithymocyte globulin (ATG) is a preparation of polyclonal antibodies frequently used to treat acute cellular rejection in organ transplant recipients. Use of rabbit ATG has been associated with serum sickness in liver and kidney transplantation patients previously exposed to rabbits. Here, we report the case of a heart transplantation patient with a history of significant rabbit exposure who had developed migratory diffuse arthralgias 13 days after receiving ATG for acute cellular rejection. Laboratory findings included C-reactive protein elevation, depressed levels of C3 and C4 complement, and strongly positive titers against rabbit immunoglobulin G, all strongly suggestive of serum sickness. To our knowledge, this is the first report of delayed serum sickness related to rabbit ATG after prior rabbit exposure in an adult heart transplantation patient. Early recognition of the symptoms of serum sickness can lead to prompt and appropriate management.

Atividade de nanoformulações de Melaleuca alternifolia e terpinen-4-ol em isolados de Rhodococcus equi / Melaleuca alternifolia activity in nanoformulations and terpinen-4-ol against Rhodococcus equi isolates

Rhodococcus equi é o agente etiológico da rodococose equina, importante doença respiratória de potros. Especialmente na última década, a emergência de cepas resistentes aos antimicrobianos empregados no tratamento da rodococose tem sido relatada. Nesse sentido, há a necessidade de estudos envolvendo terapias alternativas e novas tecnologias, incluindo o uso de plantas medicinais e nanotecnologia. Neste trabalho utilizou-se Melaleuca alternifolia nas seguintes formulações: óleo livre, nanocápsula, nanoemulsão e a combinação de óleo livre com nanocápsula e com nanoemulsão, além do seu composto majoritário, terpinen-4-ol, a fim de verificar a atividade antimicrobiana frente a isolados de R. equi de diferentes origens. Utilizou-se o método de microdiluição em caldo na determinação das concentrações inibitória mínima (CIM) e bactericida mínima (CBM) das diferentes formulações frente aos isolados (n=24). Verificou-se baixo potencial para atividade antibacteriana de M. alternifolia na formulação de óleo livre. Todavia, essa atividade foi potencializada quando se incorporou o óleo essencial às nanoformulações. O composto terpinen-4-ol demonstrou potencial atividade antibacteriana quando incorporado ao óleo essencial e quando utilizado isoladamente. Verificou-se que tanto M. alternifolia quanto terpinen-4-ol testados possuem atividade antimicrobiana contra isolados de R. equi, sugerindo seu emprego em estudos avaliando seu potencial para o tratamento da rodococose.

Rhodococcus equi causes rodococose in horses, characterized by bronchopneumonia in foals. Due to reports of antimicrobial resistance, it is important to develop studies involving alternative therapies and new technologies, including the use of medicinal plants and nanotechnology. In this work, the plant Melaleuca alternifolia in oil free formulations, nanocapsule, nanoemulsion and the combination of free and nanocapsule oil nanoemulsion, besides its major compound, terpinen-4-ol, were used in order to verify antimicrobial activity against isolates of R. equi. The broth microdilution method was employed to determine the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations of different formulations against 24 isolates. There was low antibacterial activity of M. alternifolia in oil free formulation; however, it was observed that the activity was enhanced when incorporated as essential oil the nanoformulations. The major compound, terpinen-4-ol, showed bactericidal and bacteriostatic activity when used alone. It is suggested that M. alternifolia, in association with nanocarriers systems, as well as terpinen -4-ol, presents potential for future studies concerning the equine rodococosis therapy.

Micafungin alone and in combination therapy with deferasirox against Pythium insidiosum.

This study evaluated the in vitro and in vivo activity of micafungin alone and in combination with the iron chelator deferasirox against Pythium insidiosum. Micafungin showed a poor in vitro activity when it was used alone, but synergistic interactions were observed for 88.2% of the strains when the drug was combined with deferasirox. Smaller lesions were observed in infected rabbits receiving the combination therapy, although it favored disease dissemination to the lungs. The present results show that micafungin alone is ineffective against P. insidiosum, and the combination micafungin-deferasirox might have deleterious effects for the host.

Padrões de deslocamento de bezerras de corte que receberam suplementos isolipídicos em pastagem de azevém / Displacement patterns of beef heifers receiving iso lipidic supplements on ryegrass pasture

Foram avaliados o uso de estações alimentares, o deslocamento e a taxa de bocado de bezerras de corte que foram mantidas exclusivamente em pastagem de azevém (Lolium multiflorum Lam.) ou receberam suplementos (grão de milho ou gordura). O método de pastejo foi contínuo, com número variável de animais. O delineamento experimental foi inteiramente ao acaso, com medidas repetidas no tempo. O número de bocados realizados por estação alimentar é similar quando as bezerras recebem suplemento ou não. As equações de predição mostram que a massa de lâminas foliares exerce maior influência no tempo por estação alimentar quando as bezerras permanecem exclusivamente em pastejo de azevém ou recebem grão de milho como suplemento, enquanto para bezerras que recebem gordura, essa variável é influenciada pela oferta de forragem. O deslocamento (passos entre estações e passos por minuto) de bezerras exclusivamente em pastejo e que recebem grão de milho é influenciado pela estrutura e qualidade do pasto. A taxa de bocado dos animais suplementados com grão de milho e gordura é influenciada pela proporção de lâminas foliares no dossel. Equações de regressão múltipla, considerando-se os atributos do pasto e da pastagem, podem ser utilizadas como modelos preditores do uso de estações alimentares, deslocamento e taxa de bocado de bezerras de corte...

The use of feeding stations, displacement patterns and bite rate of beef heifers kept exclusively on Italian ryegrass (Lolium multiflorum Lam) or receiving supplements (corn grain or fat) were evaluated. The grazing method was continuous with a variable number of animals. The experimental design was a completely randomized design with repeated time measures. The number of bites per feeding station is similar when heifers receive supplement or not. The prediction equations show that the mass of leaf lamina has greater influence on the time per feeding station when heifers remain exclusively grazing ryegrass or receive corn grain as a supplement, while for calves that receive fat this variable is influenced by forage allowance. The displacement (steps between stations and steps per minute) of heifers exclusively on ryegrass or receiving corn grain is influenced by the structure and quality of grass. The bite rate of heifers supplemented with corn grain and fat is influenced by the proportion of leaf lamina in the sward. Multiple regression equations considering the attributes of grass and pasture can be used as models to predict the use of feeding stations, displacement and bite rate of beef heifers...

In vitro susceptibility of zoospores and hyphae of Pythium insidiosum to antifungals.

OBJECTIVES: The purpose of this study was to compare the in vitro susceptibilities of 22 Brazilian isolates of Pythium insidiosum to antifungals using a standardized inoculum of zoospores and a proposed novel inoculum prepared from cultured mycelia (hyphae) of P. insidiosum. METHODS: A zoospore suspension of P. insidiosum was obtained by the zoosporogenesis technique. The hyphal inoculum was prepared from a suspension of P. insidiosum mycelium. Susceptibility to each drug was evaluated using the CLSI M38-A2 method. RESULTS: Of the 88 MIC comparisons performed, 36 (41%) showed the same MIC value for the two inocula. The agreement (differences not greater than one dilution) between MICs obtained with both types of inocula was 39.8% (35/88). In other MIC comparisons analysed, 17 (19.3%) showed differences of two or three dilutions. CONCLUSIONS: We conclude that the use of hyphal inocula of P. insidiosum for in vitro susceptibility tests could be a suitable method for evaluating antimicrobial susceptibility, particularly when it is not possible to obtain a standardized zoospore inoculum.

Estimation of spin-echo relaxation time.

In spin-echo-based EPR oximetry, traditional methods to estimate the T2 relaxation time, which encodes the oxygen concentration of the sample, include fitting an exponential to the peaks or the integrated areas of multiple noisy echoes. These methods are suboptimal and result in a loss of estimation precision for a given acquisition time. Here, we present the maximum likelihood estimate (MLE) of T2 from spin-echo data. The MLE provides, for the data considered, approximately 3-fold time savings over echo-integration and more than 40-fold time savings over peak-picking. A one-dimensional line search results in simple computation of the MLE. It is observed that, perhaps counter-intuitively, prior knowledge of the lineshape does not yield additional reduction of estimation error variance at practical noise levels. The result also illuminates the near optimal performance of T2 estimation via principal components calculated by a singular value decomposition. The proposed method is illustrated by application to simulated and experimental EPR data.

Dual-scan acquisition for accelerated continuous-wave EPR oximetry.

Statistical analysis reveals that, given a fixed acquisition time, linewidth (and thus pO(2)) can be more precisely determined from multiple scans with different modulation amplitudes and sweep widths than from a single-scan. For a Lorentzian lineshape and an unknown but spatially uniform modulation amplitude, the analysis suggests the use of two scans, each occupying half of the total acquisition time. We term this mode of scanning as dual-scan acquisition. For unknown linewidths in a range [Γ(min), Γ(max)], practical guidelines are provided for selecting the modulation amplitude and sweep width for each dual-scan component. Following these guidelines can allow for a 3-4 times reduction in spectroscopic acquisition time versus an optimized single-scan, without requiring hardware modifications. Findings are experimentally verified using L-band spectroscopy with an oxygen-sensitive particulate probe.

Spectral modeling for accelerated pH spectroscopy using EPR.

A data modeling and processing method for electron paramagnetic resonance (EPR)-based pH spectroscopy is presented. The proposed method models the EPR spectrum of a pH-sensitive probe in both protonated and unprotonated forms. Under slow-exchange conditions, the EPR spectrum of a sample with an unknown pH value can be accurately represented by a weighted sum of the two models, with the pH value completely determined by their relative weights. Unlike traditional pH spectroscopy, which relies on locating resonance peaks, the proposed modeling-based approach utilizes the information from the entire scan and hence leads to more accurate estimation of pH for a given acquisition time. By employing the proposed methodology, we expect a reduction in the pH estimation error by more than a factor of three, which represents an order of magnitude reduction in acquisition time compared to the traditional method.